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Guanine arabinoside were synthesized from 2,6-diaminopurine arabinoside using Pseudomonas aeruginosa TX16 cells as catalytic agent.Through optimization experiments,the reaction condition was researched.The optimal reaction conditions was obtained as follows: adding the 13% Pseudomonas aeruginosa TX16 bacteria cells,30mmol/L 2,6-diaminopurine arabinoside into the 120mmol/L pH 7.0 phosphate buffer,reaction temperature 60℃,reaction time 120 h.The conversion rate was of 95.2% at the optimal condition.