【摘 要】
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Metal homeostasis plays a critical role in anti-oxidative stress.Streptococcus oligofermentans,an oral commensal facultative anaerobe lacking catalase activity,produces and tolerates abundant H2O2,whe
【机 构】
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State Key Laboratory of Microbial Resources,Institute of Microbiology,Chinese Academy of Sciences,Be
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Metal homeostasis plays a critical role in anti-oxidative stress.Streptococcus oligofermentans,an oral commensal facultative anaerobe lacking catalase activity,produces and tolerates abundant H2O2,whereas Dpr,a Fe2+-chelating protein,dependent H2O2 protection does not confer such high tolerance.Here,we report that inactivation ofperR,a peroxide-responsive repressor that regulates zinc and iron homeostasis in Gram-positive bacteria,increased 32-fold survival of H2O2 pulsed S.oligofermentans and elevated the cellular manganese for 4.5-fold.perR complementation recovered the wild type phenotype.When growing in 0.1-0.25 mM MnCl2,S.oligofermentans increased 2.5-23-fold survival after H2O2 stress,and even higher survival was found for the perR mutant,indicating that PerR is involved in Mn2+-mediated H2O2 resistance in S.oligofermentans.
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