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Objective To screen some miRNAs binding with LPA mRNA through bioinformatics prediction and then to test the capability of lowering Apo(a) by predicted miRNAs in HepG2 cells Methods At first, we found some miRNAs with high possibility of acting on LPA expression via four forecasting tools on-line (Targetscan, miRanda, MiBD, PITA).Fluorescence detecting tested miRNA mimics transfection efficiency;RT-PCR was used to determine LPA mRNA level in HepG2 of control group and miRNA mimics groups (6 h, 12 h, 24 h);Western blot was employed to analyze Lp (a) expression level in HepG2 of groups mentioned above.Luciferase assay verified the target of miR-626.Results Bioinformatics had predicted the high possibility of regulating Apo(a)s expression miRNAs as follows: miR-655, miR-590-3p, miR-519a, miR-519b-3p, miR-338-3p, miR-519c-3p, miR-590-5p, miR-425, miR-626.Lp(a) is so high expressed in HepG2 cells that it can be used for following experiment.Experimental groups were as follows :miR-590-3p, miR-519a, miR-519b-3p, miR-338-3p, miR-519c-3p and miR-425.Experimental groups had no statistical significance in LPA mRNA level in all time point;but in protein level, miRNA-655 and miRNA-590-Sp slightly lowered Apo(a) expression;miRNA-626 significantly downregulated Apo(a) in HepG2 cells.Moreover, Apo(a) protein level in miR-626mimic + miR-626 inhibitor group is notably higher than that of miRNA-626 group and Apo(a) level in miRNA inhibitor group is higher, comparing with the control group.The cells transfected with miR-626 is lysised and the fluorescene intensity of miR-626 group is lower than the control group in target verification test.Conclusions MiR-626 can significantly downregulate the Apo(a) expression in HepG2 cells.The mechanism is that miR-626 directly bonds to 3-UTR of LPA mRNA and inhibits the translation of LPA mRNA.