Mechanical stimulus accelerate mesenchymal stem cells differentiating toward cardiomyocytes

• 作 者: Yong Guo Xizheng Zhang Chunqiu Zhang Ruixin LI Qiangcheng Zeng Chun Guo Yanjun Zhang
• 机 构: TianjinInstituteofMedicalEquipment,106WangdongRoad,Hedong,Tianjin,3001612SchoolofMechanicalEngineeri
• 出 处: Wnt信号、器官发育和干细胞国际学术大会(Wnt Signaling,Organogenesis,and Stem Ce
• 发表日期: 2009年5期
• 关键词: mechanical stimulus mesenchymal stem cells cardiomyocyte differentiation

　　Previous study demonstrated mesenchymal stem cells were sensitive to mechanical stimulus, and mechanical stimulus played great role in differentiation of mesenchymal stem cells.Therefore,mechanical stimulus maybe affect differentiation toward cardiomyocyte of mesenchymai stem cells.In this Study, the adipose tissue-derived mesenchymal stem cells were induced to differentiate with 5-azacytidine, then stimulated by 8%, 1 Hz mechanical stretch.After 10 days,the cells cardiac markers and cardiogenesis-related genes were detected by immunocytochemical staining and reverse transcriptase-polymerase chain reaction, the cells ATPase activity was also assayed.When the cells were only stimulated by mechanical stretch, αt-sacromeric actin and GATA4 were expressed at very low level, connexin43(Cx43), trial natriuretic peptide (ANP),α-myosin heavy chain (α-MHC) and Nkx2.5 were not expressed, and the cells ATPase activity was not increased.