【摘 要】
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As a main subtype of gtia in the central nervous system, astrocytes have diverse and comprehensive functions, from nutrient providing to synaptic transmission modulating.Astrocytes play protective rol
【机 构】
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State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking Uni
【出 处】
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中国神经科学学会第九届全国学术会议暨第五届会员代表大会
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As a main subtype of gtia in the central nervous system, astrocytes have diverse and comprehensive functions, from nutrient providing to synaptic transmission modulating.Astrocytes play protective roles in ischemia injury by taking up glutamate, releasing neurotrophic factors, and so on.However, the function of gap junctions in this process is still controversial.Cultured cortical neurons were treated with 3-h oxygen-glucose deprivation (OGD) to induce cell damage.We found that astrocyte-conditioned medium (ACM) could reduce neuronal death from ~95% to ~30%, as indicated by MTT assay.With centrifugal filter devices, the molecular weight of protective factors released from astrocytes was speculated to be < 3 kDa.The protection of ACM was remarkably decreased when 50 tmol/L gap junction and hemichannel blocker carbenoxolone (CBX) was applied to the cultured astrocytes before ACM collection.Furthermore, ACM pretreatment raised neuronal level of reductive glutathione (GSH) and decreased reactive oxygen species (ROS) content, while ACM pretreated with CBX could block GSH increase in neurons.These results suggested that astrocytes enhanced neuronal tolerance against OGD injury by releasing protective factors dependent on hemichannels or gap junctions.
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