3T MRI of Intracoronary Local Delivery of Motexafin Gadolinium into Coronary Artery Walls

• 作 者: Yanfeng Meng Jinnan Wang Jihong Sun Feng Zhang Patrick Willis Jiakai Li Han Wang Tong Zhang Stephanie Soriano Bensheng Qiu Xiaoming Yang
• 机 构: Department of Radiology,Taiyuan Central Hospital,Taiyuan,Shanxi,China,030009;Image-Guided Bio-Molecu
• 出 处: 第五届全国磁共振分子影像研究与应用高层论坛暨第六届山西省磁共振学术研讨会
• 发表日期: 2016年3期

　　Purpose To develop a technique,using clinical 3.0T MRI to delineate local agent distribution in coronary artery walls for potential molecular MRI-guided local gene/drug therapy of atherosclerotic coronary artery disease.Materials and methods The animal protocol was approved by the Institutional Animal Care and Use Committee and in compliance with the NIH Guide for the Care and Use of Laboratory Animals.For in vitro confirmation,we used human arterial smooth muscle cells(SMCs)to determine the capability of SMCs in uptake of Motexafin Gadolinium(MGd)and its optimal dose.For ex vivo evaluation,two-mL MGd/trypan blue mixture was locally infused into the coronary arterial walls of six cadaveric pig hearts under MRI monitoring with either an MR imaging-guidewire and/or surface coils.For in vivo validation,the agent delivery balloon was placed into the coronary arteries of seven living pigs,and MGd/blue mixture was then infused into the arterial walls under MRI.Signal-to-noise ratios(SNR)and contrast-to-noise ratio(CNR)of the coronary arterial walls using different coils between pre-and post-agent infusion were recorded,with subsequent histology confirmation.Paired student t-test was used to statistically compare the average SNRs and CNRs of arterial walls pre-and post-agent infusion with different coils.Results SMCs could uptake MGd with the optimal concentration at 150μM.Average SNR with MR imaging-guidewire and surface coil combo was significantly higher than that with MR imaging-guidewire or surface coils only(p<0.05),and average SNR and CNR of post-infusion MRI was significantly higher than that of pre-infusion MRI(p<0.05).Histology confirmed the successful intracoronary infiltration MGd/blue within the coronary arterial walls.Conclusion This study demonstrates the possibility of using MRI to delineate locally-infused agent distribution in the coronary arterial walls,which establishes groundwork for development of molecular MRI-guided intracoronary therapy.