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In this paper,we reported the significant anti-hyperglycaemic effect of the extract of Maluspumila Mil stem(MPMS)in a streptozotocin-induced diabetic mice model.The chemical composition of the extract of MPMS was analyzed by HPLC-ESI-Q-TOF.At the same time,fifteen compounds were obtained under the bioassayguided isolation strategy and determined as 6-methoxyquercetin(1),6-methoxyquercetin-3-O-α-L-rhamnopyranoside(2),8-methoxy kaempferol-7-O-rhamnopyranoside(3),quercetin-3-O-α-L-rhamnopyranoside(4),quercetin-3-Oarabinofuranoside(5),quercetin(6),quercetin-3-O-xylopyranoside(7),phloretin(8),3-hydroxy phloridzin(9),phloridzin(10),aureusidin-6-O-β-D-glucopyranoside(11),eriodictyol-7-O-glucoside(12),4-hydroxybenzoic acid(13),protocatechuic acid methyl ester(14),and cinnamic acid(15)by analysis of NMR spectroscopy.The influence of these compounds on proliferation of rat insulinoma RINm5f cells were evaluated and compound 1(quercetin-3-O-α-L-rhamnopyranoside)showed the most significant proliferation effect with the proliferation ratio values 24.45(1 μg·Ml-1).All these results demonstrate that the stems of Maluspumila Mil are useful as potential functional food ingredients for the prevention and treatment of type-2 diabetes.