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Objectives To investigate the role of endoplasmic reticulum stress in the injury induced by Bim of hypoxic cardiomyocytes.Methods The Rat cardiomyocytes, 1-3 days after birth, were cultured primarily and identified by using antibody targeting α-actin of striated muscle.Bim-siRNA were transfected transiently into cardiomyocytes by lipofectamine 2000.