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Anisakiasis caused by anisakid nematodes is an emerging infectious disease that can cause a wide range of clinical syndromes and are difficult to diagnose and treat in humans.In spite of their significance as pathogens,the systematics,genetics,epidemiology and biology of these parasites remain poorly understood.In the present study,we sequenced the complete mitochondrial(mt)genome of Pseudoterranova azarasi(sensu stricto,s.s.),which is one of the most important zoonotic anisakid parasites.The circular mt genome is 13,954 bp in size and encodes of 36 genes,including 12 protein-coding,2 ribosomal RNA and 22 transfer RNA genes.The mt gene order ofP.azarasi s.s.is the same as those ofAscaris spp.(Ascarididae),Toxocara spp.(Toxocaridae)and Anisakis simplex(Anisakidae),but distinct from those of Ascaridia spp.(Ascaridiidae)and Cucullanus robustus(Cucullanidae).Phylogenetic analyses based on concatenated amino acid sequences of 12 protein-coding genes by Bayesian inference(BI)showed that Pseudoterranova were more closely related to Anisakis than they were to Contracaecum with strong a posterior probability support.This mt genome provides a novel genetic markers for exploring cryptic/sibling species and host affiliations,and should have implications for the diagnosis,prevention and control of anisakiasis in humans.