【摘 要】
：
Our research is directed at identifying cellular components and genes that contribute to the development of atherosclerosis and restenosis.Mouse strains C57BL/6J (B6) and C3H/HeJ (C3H) are well charac
【机 构】
：
Associate Professor, University of Virginia, Radiology/Cardiovascular Research Center, USA
【出 处】
：
2005 WHTS3rd Annual Congress of International Drug Discovery
论文部分内容阅读
Our research is directed at identifying cellular components and genes that contribute to the development of atherosclerosis and restenosis.Mouse strains C57BL/6J (B6) and C3H/HeJ (C3H) are well characterized as models for diet-induced atherosclerosis.When fed a high fat/cholesterol diet containing cholate, B6 mice readily develop fatty streak lesions at the aortic root, whereas C3H mice are nearly completely resistant to lesion formation.However, interpretation of the results is complicated by differences between the two strains in plasma lipid levels and systemic inflammatory changes induced by cholate.To exclude influence from the cholate-containing diet, we have constructed a congenic strain of C3H mice carrying the apoE null allele.Despite comparable plasma lipid levels, C3H.apoE-deficient mice developed much smaller lesions than their B6.apoE-deficient counterpart.By reciprocal bone-marrow transplantation, we have demonstrated that the difference in atherosclerosis susceptibility is not associated with bone marrow-derived cells.In ex vivo stud ies, we have observed that endothelial cells are a source of differences between the two strains in atherosclerosis susceptibility.We are now performing reciprocal aorta transplantation to determine the role of the arterial wall in control of atherosclerosis susceptibility.We are also using an F2 cross to define the chromosomal locations of genes that modulate atherosclerotic lesions.
其他文献
There is no cure for AIDS at this time, although, highly active antiretroviral therapy (HAART) has greatly improved the outcome of HIV/AIDS, it is limited by side effects, viral drug-resistant, and th
The identification of tumor-specific cell surface antigens is a critical step towards the development of targeted cancer therapeutics.The epitope space at the tumor cell surface is highly complex, com
High-throughput chemical screens can be performed on intact zebrafish, making it possible to assess the effects of small molecules rapidly, inexpensively, and on a miniaturized scale.By combining the
Since the discovery of dopamine as a chemical neurotransmitter in the brain, many human neurological disorders have been linked to the malfunction of this important neural system.For example, Parkinso
Earthworm has been used as a traditional medicine to treat clotting diseases in eastern countries for centuries.In Compendium of Materia Medica edited by a Chinese traditional medical doctor Shizhen L
Recently, we have developed novel mass spectrometry for bio-complex measurements.Instead of conventional electrospray and matrix-assisted laser desorption/ionization (MALDI), laser induced acoustic de
The increasing resistance of the malaria parasites enforces alternative directions in finding new drug targets.Present findings from the malaria parasite P.vivax, causing tertiary malaria suggest euka
The recent description of the complete genomes of the two most pathogenic species of Brucella opens the way for genome-based analysis of the antigenicity of their proteins.In the present study, we des
An anti-human CD146 mouse monoclonal antibody, AA98, was generated by immunized the mouse with activated human HUVEC cells.CD146 was initially identified as a progression marker of melanoma (MUC18), a
The dominant current paradigm of drug discovery identifies potentially useful targets through one of a number of mics platform technologies.The target undergoes validation, compounds are screened and