【摘 要】
：
The identification of tumor-specific cell surface antigens is a critical step towards the development of targeted cancer therapeutics.The epitope space at the tumor cell surface is highly complex, com
【机 构】
：
Department of Anesthesia and the UCSF Comprehensive Cancer Center, University of California at San F
【出 处】
：
2005 WHTS3rd Annual Congress of International Drug Discovery
论文部分内容阅读
The identification of tumor-specific cell surface antigens is a critical step towards the development of targeted cancer therapeutics.The epitope space at the tumor cell surface is highly complex, composed of proteins, carbohydrate determinants and other post-translational modification products which are difficult to probe solely by gene expression based approaches.This epitope space can be efficiently mapped by complementary monoclonal antibodies.We have used a combinatorial human antibody library as a source of random shape repertoire to directly probe the altered surface chemistry of tumor cells.Selection conditions have been optimized to favor tumor-specific antibody binding and intemalization without prior knowledge of target antigens.We have applied this strategy to the study of prostate cancer,ovarian cancer and mesothelioma, and identified a panel of single chain antibodies that bind to internalizing receptors specifically expressed on the surface of these cancer cells.
其他文献
Process chemistry, the practice of scaling up chemical production from gram and kilograms to thousands of gallons while always of vital importance, has lately become a highly visible enterprise in the
Over the past few years significant amount of research activities in the chemical community have been directed towards the development of new technologies and methodologies for environmentally benign
Herbal drug mixtures usually contain hundreds of compounds.Some compounds exist in high concentration as major components and others exist in very low concentration as minor components.In most situati
For more than 50 years, physicians worldwide considered antibiotics as rapid and effective management of many of the most common infection of the most common conditions.According to data supplied by t
The purpose of this research is to design a mosaic protein which could be used to cure cephalophyma and cerebral edema during acute cerebral stroke.The foundation of designing this mosaic protein (TNH
Proteomics, the analysis of expressed proteins, is becoming rapidly important in the postgenomic era.A primary aim of clinical proteomics is the identification of biomarkers for diagnosis and therapeu
The anthrax lethal factor (LF),a Zn-dependent endopeptidase,has a major role in the development and virulence of anthrax,an infectious disease caused by the toxigenic bacterium Bacillus anthracis. Con
Basic fibroblast growth factor (bFGF) and its application in accelerating acute wound healing.In general, acute wound healing process is convenient to divide the overall into three overlapping phases:
Microtubule-binding protein Tau is critical for neuronal function.Expression and function of human tau gene are under complex regulation by alternative splicing.Exclusion or inclusion of exon 10 leads
There is no cure for AIDS at this time, although, highly active antiretroviral therapy (HAART) has greatly improved the outcome of HIV/AIDS, it is limited by side effects, viral drug-resistant, and th