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Objective To investigate the molecular mechanism of endosulfan reproductive toxicity.Methods The GC-lspg cells were exposed to 0,6,12 and 24μg/mL endosulfan for 24h,respectively.Cell viability was determined by MTT assay.The LDH release and the malondialdehyde (MDA) content were measured by enzyme-linked immunoassay.Apoptosis in GC-1 spg cells,cell cycle and proliferation were determined using flow eytometry.