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AIM: To use soya bean root to separate and extract a tumor-activity resistant substance from SHANDOUGEN and probe its effect mechanism METHODS: Take a Sun-dried sample of medicinal soya bean roots, and use the dissolution method: first Extract the substance to be probed and determine its activity; use HP-20 gel open column clromatography to separate; Further use HPLC in preparation; finally obtain 3 chemical compounds that have a distinct increased inhibitory effect in among other places HL60Leukemia cells, use MAS ,NMR etc machinery to analyses and determine the chemical structure of these compounds, use MTT determination method, Fluorescent, color method to observe the change in the structure of the nucleus, Electrophoresis method to carry out fragment DNA inspection as well as active oxygens inter-relation test among other tests to carry out the probe..