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目的:研究逍遥散对慢性应激大鼠海马神经元内Ca2+浓度及NR1 mRNA表达的影响,探讨逍遥散对应激损伤学习记忆保护的作用机制。方法:采用慢性多相性应激大鼠模型,以Fura-2负载及荧光分光光度计检测大鼠海马突触体内Ca2+浓度,RT-PCR法检测海马神经元内NR1 mRNA表达的变化。结果:模型组大鼠海马突触体内Ca2+浓度及NR1 mRNA表达均明显上升(P<0.01),而与模型组比较,逍遥散组大鼠突触体内Ca2+浓度及NR1 mRNA表达明显回落(P<0.05,P<0.01)。结论:抑制慢性应激所导致的大鼠海马神经元内NR1 mRNA的过度表达,减少神经细胞膜上NR的数量,从而减轻慢性应激状态下海马神经元内Ca2+超载所致的细胞毒性损伤。这可能是逍遥散抗应激损伤的关键机制。
Objective: To investigate the effects of Xiaoyaosan on Ca2 + concentration and NR1 mRNA expression in hippocampal neurons of rats with chronic stress, and to explore the mechanism of Xiao Yao San on the protection of learning and memory induced by stress. Methods: Ca2 + concentration in rat hippocampal synaptosomes was detected by Fura-2 load and fluorescence spectrophotometer. The expression of NR1 mRNA in hippocampal neurons was detected by RT-PCR. Results: Ca2 + concentration and NR1 mRNA expression in hippocampal synapse of rats in model group were significantly increased (P <0.01), while compared with model group, Ca2 + concentration and NR1 mRNA expression in synaptic cleft of rats in Xiaoyaosan group were significantly decreased (P < 0.05, P <0.01). CONCLUSION: It can inhibit the overexpression of NR1 mRNA in rat hippocampal neurons caused by chronic stress, and reduce the amount of NR on the membrane of neurons, thereby reducing the cytotoxic injury induced by Ca2 + overload in hippocampal neurons under chronic stress. This may be the key mechanism for Xiaoyaosan to resist stress injury.