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Transposable insertion is an important tool for generating mutant populations that can be used for forward genetic screen.Transposon mutagenesis results in multiple random insertions and thus it is important to identify most,if not all,insertion sites in the genome.These insertion sites provide candidate causal mutations for desired phenotypes for further functional validation.Traditional means such as TAIL-PCR are time consuming and labor-intensive,and cannot recover most insertion sites.As the cost of next-generation sequencing becoming lower and lower,it is efficient and cost-effective to use genome re-sequencing data to identify transposon insertion sites.