【摘 要】
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In the present study,a fast method using high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry was developed to determine the contents of oxypaeoniflorin
【机 构】
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Department of Integrative Medicine,Huashan Hospital,Fudan University,Shanghai,200040,China
【出 处】
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第三届民族传统医学与现代医学国际学术大会暨第十三次全国中西医结合防治呼吸系统疾病学术研讨会
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In the present study,a fast method using high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry was developed to determine the contents of oxypaeoniflorin,albiflorin,paeoniflorin,benzoylpaooniflorin,baicalin,wogonoside,oroxylin A-7-O-glucuronide,baicalein,wogomn,and oroxylin A in Qingrehuoxue decoction,which has been used for the treatment of COPD.Separation was performed on a Poroshell 120-C18 reversed-phase column.The total run time was 18 min.Gradient elution was applied using a mobile phase of 0.1% formic acid in 5 mM ammonium/acetonitrile.The analytes were quantified on an Q-TOF mass spectrometer in electrospray ionization MS mode.It was found that the calibration curves for all analytes showed good linearity (R2>0.99) within the test ranges.The relative standard deviations (RSDs) for intra-and inter-day precisions were below 3.2% and 7.4%,respectively.The accuracy was evaluated by the recovery test within the range of 82.57% to 104.78% with RSD less than 5.0%.The sample was stable for at least 48 h at 4℃.The results showed that the new approach is accurate,sensitive,and reliable,and has been successfully quantified the 10 major constituents in three batches of Qingrehuoxue decoction.
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