【摘 要】
:
目的:本文考察了贵州省滇重楼资源的分布情况,并对滇重楼药材品质进行评价,为贵州这一资源的合理利用、栽培驯化研究提供理论依据.方法:样品收集采用实地调查、自采及集市采购;采用HPLC.法对滇重楼重楼皂苷Ⅰ,Ⅱ,Ⅵ,Ⅶ进行测定.色谱柱为Waters C18柱(4.6 mmx250mm,5μm),流动相为乙腈(A)-水(B)梯度洗脱,0~40min (30%~60%A),40~50min(60%~30%
【机 构】
:
安顺学院 安顺 561000 重庆市食品药品检验所 重庆 401121
【出 处】
:
第三届民族传统医学与现代医学国际学术大会暨第十三次全国中西医结合防治呼吸系统疾病学术研讨会
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目的:本文考察了贵州省滇重楼资源的分布情况,并对滇重楼药材品质进行评价,为贵州这一资源的合理利用、栽培驯化研究提供理论依据.
方法:样品收集采用实地调查、自采及集市采购;采用HPLC.法对滇重楼重楼皂苷Ⅰ,Ⅱ,Ⅵ,Ⅶ进行测定.色谱柱为Waters C18柱(4.6 mmx250mm,5μm),流动相为乙腈(A)-水(B)梯度洗脱,0~40min (30%~60%A),40~50min(60%~30%A),检测波长为203 nm,柱温25℃,流速1.0mL·min-1,进样量10 μL.
结果:贵州省滇重楼资源以安顺地区西经黔西南州至云南曲靖这一条带为分布中心;含量测定结果表明绝大多数药材样品满足药典要求,贵定县都六乡的样品2、产自兴义市乌沙镇的样品5以及普定县鸡场乡的样品7三个品质最好,4种皂苷总含量超过2%.
结论:贵州省滇重楼品质良好而稳定,其种质资源对比与其他产地具有极大的优势.
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