【摘 要】
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Aim Baicalein (BA), one of the major compounds isolated from the root of Scutellaria baicalensis Gerogi, is a bioactive flavonoid with various pharmacological effects.Herein, wed like to determine whe
【机 构】
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State Key Laboratory of Quality Research in Chinese Medicine,Institute of Chinese Medical Sciences,U
【出 处】
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2015医学前沿论坛暨第十四届全国肿瘤药理与化疗学术会议
论文部分内容阅读
Aim Baicalein (BA), one of the major compounds isolated from the root of Scutellaria baicalensis Gerogi, is a bioactive flavonoid with various pharmacological effects.Herein, wed like to determine whether BA triggers autophagy in ovarian carcinoma HEY cells and the involved mechanisms.Methods Cell proliferation was determined by MTT assay.BA-triggered autophagy was measured by monodansylcadaverine (MDC) staining and the expression of lipid modification of light chain-3 (LC3), respectively.SiRNA and pharmacological regulators were used to study the mechanisms of BA-triggered-autophagy.Results BA inhibited the proliferation on HEY cells after 24 h treatment.Meanwhile, BA triggered the acidic vesicular organelle and enhanced the expression of LC3II in HEY cells.Inhibition of autophagy by chloroquine (CQ), an inhibitor of autophagy, the cell viability was obviously decreased whereas the expression of cleaved PARP was significantly increased, indicating that BA triggered a protective autophagy.We also found siRNA Beclin 1 obviously reversed BA-induced LC3II expression.Furthermore, pretreatment with U0126, an MEK inhibitor, or siRNA of ERK, obviously inhibited BA-induced activation of ERK as well as expression of LC3II.Conclusion BA triggers a protective autophagy via Beclin 1 and ERK dependent manners in ovarian carcinoma HEY cells.
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