【摘 要】
：
Objective: This study is designed to investigate whether the Müller glial cells in diabetic retinopathy participates in the formation of retinal fibrosis and define the signaling pathways and key mole
【机 构】
：
Department of Biochemistry,Zhongshan School of Medicine,Sun Yat-sen University,Guangzhou,510080
【出 处】
：
中国生物化学与分子生物学会2016年全国学术会议
论文部分内容阅读
Objective: This study is designed to investigate whether the Müller glial cells in diabetic retinopathy participates in the formation of retinal fibrosis and define the signaling pathways and key molecular involved.Finally,the possible mechanism for the pathological changes of Müller glial cells are discussed.Methods:(1)After 6-8 weeks high-fat feeding,type 2 diabetic mellitus models were prepared with low dose STZ(20mg/kg)intraperitoneal injection in male C57BL/6J.And db/db mice was used as type 2 diabetic model as well.The levels of mesenchymal markers were determined by immunoblotting and immunofluorescence.(2)In vitro,Müller cells were cultured in 25 mM D(+)-glucose medium and mannitol medium respectively.The levels of mesenchymal markers,N-cadherin,β-catenin and Snail were determined by immunoblotting and immunostaining.Then the Wound healing assay was applied to evaluate the migratory ability of Müller cells.(3)A vector encoding SNAI1 and si-SNAI1 were transfected into the cells respectively.The levels of fibrogenic factors,fibronectin,GFAP and CTGF were determined by immunoblotting.Results:(1)The levels of mesenchymal markers,N-cadherin,Snail,Vimentin,β-catenin,α-SMA and Twist were increased in type 2 diabetic models.(2)High glucose elevated the levels of mesenchymal markers,N-cadherin,β-catenin and Snail,while promoted the migration as well.(3)Overexpression of Snail elevated the fibronectin and connective tissue growth factor(CTGF)levels while silencing Snail attenuated the over-expression of fibronectin and CTGF induced by high glucose in cells.Conclusion: Up-regulation of mesenchymal markers in Müller cells and diabetic retina suggest that mesenchymal transition,may represent a new pathogenic mechanism in DR fibrosis formation.
其他文献
我国是芥菜型油菜起源地之一，资源丰富。种子颜色既是植物驯化现状，又是重要的遗传标记性状与作物品质的指示性状。本研究以芥菜型油菜黄色品种“四川黄籽”及其近等基因系黑籽油菜和甘蓝型油菜黑色品种及芥甘杂交后代黄色和黑色品种种质为材料，通过植株生长发育与农艺性状观测、不同阶段种子发育的解剖结构与特性、植物激素含量和种籽发育基因表达分析等，以揭示黄籽油菜的生物学特性。
兴安落叶松(Larix gmelinii)隶属于松科落叶松属，是中国东北、华北、西南及内蒙古地区主要的落叶乔木，其木材挺直、纹理清晰、材质致密，具有较强的机械性能，适用于做室内装饰板、家具和人造板，具有很高的经济价值，对其生长发育及木材形成调控的研究具有重要意义。
海藻糖是一种非还原性双糖,胁迫条件下可作为应激保护剂,对蛋白质和生物膜起到保护作用,海藻糖含量的升高能够增强植物对各种非生物胁迫的耐受性,具有保存生物活力的特殊功能。海藻糖-6-磷酸合酶(Tps)与海藻糖-6-磷酸磷酸酯酶(Tpp)是海藻糖代谢途径中的关键酶,Tps 和Tpp 基因的表达直接影响海藻糖的合成。
构树(Broussonetia Papyrifera(L.)Vent)为桑科(Moraceae)构树属的多年生落叶乔木；具有生长快、适应性强、分布广、易繁殖、抗污染能力强、耐烟尘污染等特点，被广泛应用于造纸、饲料、医药等行业，并在扶贫产业、环境保护生态修复上发挥重要作用.在高等植物中，多糖代谢在调控纤维素、半纤维素、果胶和胼胝质等细胞壁物质的形成、营养器官的生长及生殖器官的发育过程发挥重要作用.
王枣子为香茶菜属植物，具清热解毒、抗菌消炎等功效。目前国内外对香茶菜的研究主要集中在化学成分的提取，鉴定以及药理作用方面，对其外植体的消毒方法和打破休眠的基础性研究很少。因此本文选取成熟的王枣子种子，于11月份至次年2 月份对其外植体的消毒和种子打破休眠的方法进行研究。研究发现70％乙醇消毒1min、升汞消毒10min 的王枣子种子污染率低且萌发率高；70％乙醇消毒30 s、升汞消毒8 min 的
The desiccation tolerant desert moss Syntrichia caninervis is a potential source for genetic element that could be utilized for crop improvement for drought tolerance.An ABSCISIC ACID INSENSITIVE3(ABI
The endosomal sorting complex required for transport(ESCRT)machinery is an ancient system that mainly controls multivesicular body(MVB)biogenesis and MVBmediated membrane protein sorting.Although the
Aneuploidy,defined as unequal numbers of different chromosomes,is a large-effect genetic variant and may produce diverse cellular and organismal phenotypes.Polyploids are more permissive to chromosoma
Ethylene production has been achieved in cyanobacteria by introducing an exogenous ethylene forming enzyme(EFE)that using the TCA intermediate 2-oxoglutarate(2-OG)as one of its substrates.NtcA is a gl