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The major feather of Parkinsons disease (PD) is the loss of dopaminergic (DA) neurons in substantia nigra pars compacta (SNc).DA-denervation induces a complex rearrangement within the basal ganglia circuitry and profound modifications in the activity of the glutamatergic pathways.Therapeutic approaches aiming at the reduction of the increased activity of the corticostriatal synaptic transmission after DA-denervation is likely to act as a new strategy for PD.Previous studies have shown that high frequency electroacupuncture (EA) stimulation can improve symptoms in PD animal models.However, the mechanism is still unclear.In this study, the effects of EA on behavior and the dopamine contents were tested in 6-hydroxydopamine (6-OHDA) lesioned rats.Glutamate (Glu) release were conduted by microdialysis and HPLC, and mGluRs expression was also tested in the striatum.100 Hz EA effectively improved the motor symptoms including rotational and rotarod behaviors.Neither dopaminergic neurons in SNc nor DA content in the striatum have been found to be normalization by EA treatment.Glu levels in the striatum significantly increased after 6-OHDA lesion, but the increased Glu release were reversed by EA stimulation.EA downregulated the expression of metabotropic glutamate receptor (mGluRs)2/3 in the striatum.Meanwhile, administration of the selective antagonist of groupⅡ mGluRs (APICA) proved that the effects of behavorial improvement induced by EA have disappeared.These results suggested that highfrequency EA stimulation improves motor impairment by decreasing corticostriatal glutamatergic transmission in 6-OHDA-lesioned rats.