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In this study,riboflavin (RF) was immobilized on graphite felt anode by electropolymerization of pyrrole while using RF as the dopant.The modified anode was demonstrated to enhance the simultaneous Congo red decolorization and bioelectricitygeneration in air-cathode microbial fuel cell (MFC).Immobilization of 0.5 C and 1.25 C RF resulted in 20.0 % and 41.4 % increase in maximum power density,respectively.The data of reduction in anode polarization resistance and accelerated reductive decolorization of Congo red indicated that immobilization of RF in the anode is particularly effective in facilitate electrons transfer from microbes to azo dye and microbes to anode.Evidence from cyclicvoltammerty (CV) and electrochemical impedance spectroscopy (EIS) results indicated that the increased power generation and accelerated Congo red decolorization was attributed to the RF immobilized to the anode,and the anode performance was positively correlated with immobilized quantity of RF.