【摘 要】
：
Acacetin (5,7-dihydroxy-4-methoxyflavone), a flavonoid compound, has anti-peroxidative and anti-inflammatory effects.The effect of acacetin on antimetastasis in human prostate cancer DU-145 cells was
【机 构】
：
Department of Surgery, Chi Mei Medical Center Taiwan
【出 处】
：
BITs 3rd Annual World Cancer Congress-2012(2012第五届世界癌症大会)
论文部分内容阅读
Acacetin (5,7-dihydroxy-4-methoxyflavone), a flavonoid compound, has anti-peroxidative and anti-inflammatory effects.The effect of acacetin on antimetastasis in human prostate cancer DU-145 cells was investigated.First, the result demonstrated acacetin could exhibit an inhibitory effect on the abilities of the adhesion, invasion, and migration by cell-matrix adhesion assay, wound-healing assay, and Boyden chamber assay.Data also showed acacetin could inhibit the phosphorylation of p38 mitogen-activated protein kinase (p38 MAPK) involved in the downregulation of the expressions of matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMp-9), and urokinase-type plasminogen activator (u-PA) at both the protein and mRNA levels.Next, acacetin significantly decreased the nuclear levels of nuclear factor kappa B (NF-kappaB), c-Fos, and c-Jun.Also, the treatment with acacetin to DU145 cells also leads to a dose-dependent inhibition on the binding ability of NF-kappaB and activator protein-1 (AP-1).Furthermore, the treatment of inhibitors specific for p38 MAPK (SB203580) to DU145 cells could cause reduced expressions of MMP-2, MMP-9, and u-PA.These results showed acacetin could inhibit the invasion and migration abilities of DU145 cells by reducing MMP-2, MMP-9, and u-PA expressions through suppressing p38 MAPK signaling pathway and inhibiting NF-kappaB-or AP-1-binding activity.These findings proved acacetin might be offered further application as an antimetastatic agent.
其他文献
We discovered an immunogenic 90kD glycoprotein tumor-associated antigen (TAA), and developed a murine monoclonal antibody based ELISA to detect TAA specific immune complexes (IC) in sera of breast can
The urokinase plasminogen activator (uPA) system comprises the serine protease uPA, its receptor uPAR and two inhibitors PAI-1 and PAI-2.Components of the uPA system have an important role in tumorige
The majority of smooth muscle tumors found in the uterus are benign, but uterine leiomyosarcoma (LMS) are extremely malignant, with high rates of recurrence and metastasis.The development of gynecolog
Clinicians often experience extrahepatic metastases associated with hepatocellular carcinoma (HCC), even if no evidence of intrahepatic recurrence after treatment is observed.We investigated the pretr
Triple Negative Breast Cancer (TNBC) is an aggressive subtype of breast cancer (BC) that lacks the expression of estrogen receptor, pro.gesterone receptor and human epidermal growth factor receptor-2.
The POU domain transcription factor Oct4 (POU5F1 or Oct3) is a member of the homeobox gene family and is specifically expressed in totipotent mouse and human embryonic stem (ES) and germ cells.It is c
In a single cell, the genome consists of individual DNA molecules of specific sequences.In cancers, genomic instability results in variations of DNA among cells originating from the same lineage.Singl
Soft tissue angiofibroma is a recently delineated tumor type of unknown cellular origin.Cytogenetic analysis of four cases showed that they shared a t(5;8)(p1 5;q1 3).In three of them it was the sole
DNMT methyltransferase 1 (DNMT1) plays a significant role in maintaining DNA methylation status.Aberrant DNA methylation is a recognized feature of human cancers, and folate is directly involved in DN
Activation of hypoxia signaling pathways is strongly associated with poor prognosis in cancer.Inactivation of the tumor suppressor gene encoding fumarate hydratase causes activation of hypoxia signali