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In a single cell, the genome consists of individual DNA molecules of specific sequences.In cancers, genomic instability results in variations of DNA among cells originating from the same lineage.Single cell whole-genome amplification and sequencing is highly desirable in order to characterize individuality and heterogeneity among cells.However,existing amplification methods are hindered by nonuniformity across the genome and the introduction of spurious sequences.We report a new amplification method which circumvents these problems by performing linear pre amplification with Multiple Annealing and Looping Based Amplification Cycles (MALBAC), before exponential amplification with PCR.We demonstrate sequencing single human cells with ~93% coverage at ~30x depth.Individual cells in a cancer cell line exhibit distinctly different and digitized copy number variations, which are otherwise masked in bulk sequencing.Filtering out amplification errors by sequencing two daughter cells, we demonstrate definitive detection of unique single nucleotide variations in a single cancer cell.