【摘 要】
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Background: The free beta subunit of human chorionic gonadotropin (free beta-hCG) is an important serum marker for biochemical screening.Its weekly median value varies with ethnicity.Most of the fluor
【机 构】
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Prenatal Diagnosis Centre, Guangdong Women and Children Hospital, Guangzhou, Guangdong, China
【出 处】
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广东省遗传学会第九届代表大会暨学术研讨会
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Background: The free beta subunit of human chorionic gonadotropin (free beta-hCG) is an important serum marker for biochemical screening.Its weekly median value varies with ethnicity.Most of the fluorometers for lanthanide chelates are designed for the detection of signals from europium (Eu(3+)) chelates only.Methods: We developed a two-site, one-step assay using two monoclonal antibodies (Mabs) against free beta subunit and beta subunit with Eu(3+) chelates as labels.Using the present assay, we evaluated 24,634 normal serum samples in Chinese pregnant women during 8-20 weeks of gestation.Results: The detection limit using this assay was <0.05 ng/mL.The within-run and between-run imprecision was <6.0% and 7.0% using control material.Free beta-hCG concentrations measured using the current assay in 999 maternal serum samples correlated well with those obtained by samarium (Sm(3+))-labeled DELFIA free hCGbeta assay (r=0.987).The medians for 8-20 weeks for maternal serum free beta-hCG were higher in the women from mainland China compared to reports from other countries.Conclusions: The present assay is suitable for use in biochemical screening of women in mainland China.Our study on the median concentrations of free beta-hCG will help establish reference values that are specific for ethnic populations from the Chinese mainland.These will be useful for studying the importance of ethnic factors in biochemical screening.
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