【摘 要】
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Stimulated emission depletion(STED)nanoscopy with a high lateral spatial resolution(< 50 nm)has provided a powerful sub-diffraction imaging tool to probe the most relevant subcellular structure and or
【机 构】
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Beijing Key Laboratory for Optical Materials and Photonic Devices,Capital Normal University,School o
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Stimulated emission depletion(STED)nanoscopy with a high lateral spatial resolution(< 50 nm)has provided a powerful sub-diffraction imaging tool to probe the most relevant subcellular structure and organelles.[1] Recently,various luminescent probes have been developed and successfully applied to STED nanoscopy such as QDots,perovskite QDs,AIE nanoparticles,upconversion nanoparticles(UCNPs),commercially organic dyes and so on.[2] Herein,we developed highly-emissive silica-coated core-shell ONPs(CSONPs)as a new type of photostable probes with ultrahigh stimulated emission depletion efficiency(99%)for low-power super-resolution STED nanoscopy.A nonplanar twist intramolecular charge transfer(TICT)compound((E,E)-(2,5-bis(diphenylamino)-1,4-phenylene)bis(ethene-2,1-diyl))bis(3-fluorobenzonitrile)(DAPF)was designed and synthesized to prevent effective π-π interaction and excimer formation in the solid state,offering our CSONPs with i)high solid-state fluorescence quantum yields around 0.6,ii)large Stokes shift of 150 nm and iii)high photostability owing to silica shell protection.
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