【摘 要】
：
表面增强拉曼光谱(SERS)技术可以从分子水平给出化合物的分子结构信息,是一种高灵敏的生化检测技术.拉曼增强基底的制备是SERS的技术核心,现有的SERS基底制备方法很多,其
【机 构】
：
重庆大学化工学院,中国,重庆,400030;新型微纳器件与系统技术国防重点学科实验室,中国,重庆,400030;重庆大学微系统研究中心,中国,重庆,400030;四川理工学院分析测试中心,中国,四川,
【出 处】
：
第九届全国微全分析系统学术会议、第四届全国微纳尺度生物分离分析学术会议、2014国际微流控芯片与微纳尺度生物分离分析学术
论文部分内容阅读
表面增强拉曼光谱(SERS)技术可以从分子水平给出化合物的分子结构信息,是一种高灵敏的生化检测技术.拉曼增强基底的制备是SERS的技术核心,现有的SERS基底制备方法很多,其中电化学沉积是一种最为有效的SERS基底制备途径.本文报道了电化学沉积ITO-nano-Ag纳米膜基底,用PDMS在基底表面构建微孔阵列,制备ITO-nano-Ag SERS芯片的方法.实验首先采用三电极体系,以ITO导电玻璃为工作电极,铂电极为对电极,饱和苷汞电极为参比电极,在含0.01M硝酸钾支持电解质的银氨溶液为电解液体系中,用双电位计时电位沉积技术在ITO导电玻璃表面沉积制备形貌可控nano-Ag SERS基底.用罗丹明6G为探针分子研究了沉积电位、沉积时间和银氨溶液浓度对SERS效果的影响,用SEM和XRD对纳米银的组成和形貌进行了表征.结果表明沉积电位和沉积时间对纳米银的结构和SERS效果有很大影响,在-1.4V沉积30s,-0.2V沉积300s条件下制备的棒状纳米银结构的SERS增强效果最好,对R6G的最低检测浓度为10-10M,以R6G1642cm-1的吸收峰计算增强因子为4.36×106.该nano-Ag 表面增强(SERS)基底用于牛血清蛋白测定,能显著增强牛血清白蛋白的拉曼信号,对牛血清蛋白的最低检测浓度为1×10-7M.然后用带有6个2μm孔径的微孔阵列的PDMS膜覆盖在ITO-Ag纳米膜表面,再在PDMS表面覆盖石英玻璃形成具有6个微孔检测窗口的简易nano-Ag SERS微阵列芯片,每个微孔为一个封闭的检测窗口,可减小拉曼激光照射样本挥发,固定基底表面样本的液层厚度,提高SERS测试的信号稳定性和重现性.
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