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In this study,a combined proteomic and transcriptomic analysis was performed to understand the mechanisms underlying the immunomodulation induced by recombinant galectins of Haemonchus contortus(rHco-gal-m/f)on goat peripheral blood mononuclear cells(PBMC).We demonstrated that rHco-gal-m/f could be distinguished by antisera from goats experimentally infected with H.contortus and bound to the surface of goat PBMC.