Prokaryotic expression and reactivity analysis of serine proteinase inhibitor gene of Trichinella sp

• 作 者: Nian-ZhangZHANG[1]Wen-YahGAI[1]Wen-HuiLI[1]Hong-BinYAN[1]Zi-GangQU[1]Jian-MinCUI[1]Bao-QuanFU[2]
• 机 构: State Key Laboratory of Veterinary Etiological Biology,Key Laboratory of Veterinary Public Health of
• 出 处: 中国畜牧兽医学会兽医寄生虫学分会第十三次学术研讨会
• 发表日期: 2015年期

　　The specific primers derived from T.spiralis serine proteinase inhibitor(TsSerPIN)gene in GenBank database were designed and used to amplify the TsSerPIN gene from total RNA isolated from T.spiralis muscle larvae.The RT-PCR Produet was ligated into the expression vector pET30a and recombinant plasmid was transformed to E.coli BL21(DE3)and induced by IPTG for expression.