【摘 要】
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The sex-specific DNA markers applicable to fish were very useful for elucidating the sex determination mechanism and sex control in fish.In the present study, amplified fragment-length polymorphism (A
【机 构】
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Key Lab of Mariculture and Enhancement of Zhejiang province, Marine Fishery Institute of Zhejiang Pr
【出 处】
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第十届长三角科技论坛水产科技分论坛暨2013年浙江省渔业科技论坛
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The sex-specific DNA markers applicable to fish were very useful for elucidating the sex determination mechanism and sex control in fish.In the present study, amplified fragment-length polymorphism (AFLP) approach with 144 primer combinations was employed to identify sex-specific markers in the rock bream.Four male-specific AFLP fragments were identified which were designated as Op1286, Op1237, Op1422, and Op1228.Further sequencing analysis of the sex-markers genomic region revealed subtle differences between the males and females.We identified 4 male-specific single nucleotide polymorphisms (SNPs) and a deletion of 8 bp in marker Op1286, 6 male-specific SNPs in marker Op1237, 3 male-specific SNPs in marker Op1422, and 8 male-specific SNPs and 1 bp inversions in marker Op1228.Specific primers were designed based on the nucleotide variations in the sequences to develop a simple polymerase chain reaction (PCR) method for identifying genetic sex of rock bream.As a result, three out of the four male-specific markers were converted into SNP markers.The male-specific AFLP markers and AFLP-derived SNP markers were tested in 100 individuals collected from three locations around the coast of Zhoushan, giving reproducible sex identification.These male-specific DNA markers will provide an important tool for the identification of sex-determining locus and artificial breeding in the rock bream.
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