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Mycobacterium tuberculosis PPE68 gene from the total DNA of Mycobacterium tuberculosis was am plified and subcloned into eukaryotic expression vector pcDNA3.1 (+)for extraction of recombinant antigen and immunogenicity analysis.The total DNA of Mycobacterium tuberculosis was extracted and the PPE68 gene was amplified by PCR.The DNA fragment was subcloned into eukaryotic expression vector pcDNA3.1 (+)following the insertion and amplification in pGEM-T.The recombinant plasmid was transfected into HeLa cells and ex pression products was identified by Western blot.