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Content: The bacterial type Ⅳ pilus (T4P) is the strongest biological motor known to date as its retraction can generate forces well over 100 pN.Myxococcus xanthus, a δ-proteobacterium, provides a good model for T4P investigations because its social (S) gliding motility is powered by T4P.In this study, the interactions among M.xanthus T4P proteins were investigated using genetics and the yeast two-hybrid (Y2H) system.