胚胎发育晚期丰富蛋白(Late Embryogenesis Abundant Protein,LEA)是一类普遍存在于动物、植物和微生物中与渗透调节相关的特异家族蛋白.该类蛋白富含亲水氨基酸,是一类稳定的亲水性蛋白,在生物耐受干旱和盐等非生物胁迫过程中起重要作用.
α-淀粉酶(EC 3.2.1.1)从淀粉糖链内部水解1,4-α-D-葡萄糖苷键,是工业中应用最广的酶制剂之一.具有高催化效率和碱稳定性的α-淀粉酶在纺织业和洗涤业中具有潜在的应用价值,已成为目前酶制剂研究领域的一个重要方向.
从湖南小溪国家级自然保护区采集非盐环境(ordinary non-saline environment)土壤样品,用纯培养法(culture-dependent method)分离其中的嗜盐和耐盐原核生物,进而采用基于16S rRNA基因序列的系统发育分析方法研究这些分离菌株的多样性,并对部分代表潜在新分类单元的菌株进行系统分类学研究.
本文构建了转大蒜金属硫蛋白基因(MT)的工程酵母菌株,其中MT的表达受铜离子或甲醇诱导型启动子调控.工程菌过表达MT可增强菌体对有害重金属离子铅、砷、铬、镉的耐受性,提高细胞对环境有害重金属的适应性;用高表达MT的菌体处理重铬酸钾配制的模拟废水,采用固定化菌体或裸菌处理,发现转MT工程菌对铬离子的去除效果明显好于对照菌,菌体投加量和作用时间对铬离子去除率有明显影响,菌体投加量增加能增强对溶液中铬离
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