【摘 要】
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Scorpion venom constitutes a vast library of neurotoxic peptides which can provide useful probes for exploring ion channels functions.It is estimated that more than 100,000 peptide toxins exist in 150
【机 构】
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Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chines
【出 处】
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第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会
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Scorpion venom constitutes a vast library of neurotoxic peptides which can provide useful probes for exploring ion channels functions.It is estimated that more than 100,000 peptide toxins exist in 1500 distinct scorpion species, but only a very small portion (less than 0.02%) of these peptides has been identified.Peptides purification is still the most common way to study the components of crude venom.Current purification starts with gel filtration, followed by successive SCX, sometimes by fast protein liquid chromatography, and finally RPLC.However, this approach is strenuous, low efficiency, and not suitable for large-scale purification.For the discovery of novel potential toxic peptides, this requires first the development of techniques that allows for the purification of toxins from highly complex samples.
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