Influence of RNA Interference Mediated Cyclin D1 Gene Silencing on The Proliferation and G1 Phase Re

• 作 者: Dongmei;LijunHao;
• 机 构: Plastic and Cosmetic Center, The First Affiliated Hospital of Harbin Medical University, 23 Youzheng
• 出 处: 2014第6届中国国际美容医学大会、第二届美容整形院长及科主任论坛、两岸医疗美容学术论坛、中韩美容医学交流会、第十届口腔
• 发表日期: 2014年期

　　In order to investigate the effect of sequence-specific small interfering RNA on suppressing cyclin D1 expression and proliferation and cell cycle and expression of G1 phase regulators of fibroblasts derived from keloid, the plasmid expression vector of siRNA targeted against cyclin D1 was constructed and transfected into fibroblasts with Lipofectamine TM2000.The changes of cyclin D1 expression were detected by fluorescent quantitative PCR(FQ-PCR), semi-quantitative RT-PCR.The effect of sequence-specific small interfering RNA in suppressing the proliferation of fibroblasts was detected by MTT assay.Flow cytometry were used for evaluation of cell cycle.The expression of cyclin D1, CDK4, pRb and P16 was detected by immunohistochemical method.