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Cells reside in a highly complex niche in vivo composed of soluble factors,cell-matrix interactions,and cell-cell contacts,while traditional cell culture techniques have been limited by their inability to fully capture nuances of the in vivo cellular microenvironment.Microfluidic devices provide the capability of mimic the natural cell physiological experimental platforms that incorporate multi-parameter [1-2].To quantify the response of axons to gradient signals differed in steepness,we proposed a novel laminar-based microfluidic device enabling generate gradients with multiple different steepness simultaneously in a single microfluidic chip; To combine cell culture and real-time detection,we developed an integrated microdevice which integrates long-term cell culture and microelectrode array for electrochemical detection[3]; Reproducing the physiological environment of blood vessels for the in vitro investigation of endothelial cell functions is very challenging.We described a vascular-like structure based on a three-dimensional gelatin chip and TiC/C nanowire arrays microelectrode for highly-sensitive nitric oxide detection[4]; To in vitro study tumor metastasis and angiogenesis,we developed a novel artificial blood vessel implanted three-dimensional collagen chip model for reconstitution of tumor metastasis and angiogenesis.In addition,we proposed an approach to generate hydrogel-based fine vascular network models using chitosan as sacrificial template.