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目的:评价糖心安胶囊对糖尿病神经病变的防治作用。方法:雌性小鼠一次性腹腔注射链脲佐菌素150mg/kg以复制糖尿病模型,将糖尿病小鼠随机分为模型组、卡马西平0.1 g/kg、糖心安0.45 g/kg、糖心安0.15 g/kg、糖心安0.05 g/kg。灌胃给药,每天一次,连续14天,模型组和正常对照组分别给于等体积0.5%羧甲基纤维素钠,于末次给药后1小时测定其热痛阈值、压痛阈值、血糖水平、Aδ纤维以及C纤维敏感性等指标。结果:Ⅰ型糖尿病小鼠热痛阈值、机械刺激痛阈值降低,在CS条件下(30V),C纤维对电刺激产生明显高反应性;在post-CS条件下(15V),Aδ纤维在强电刺激后对低电刺激的易化(facilitation)程度明显降低;糖心安0.15 g/kg、0.45 g/kg可显著增加糖尿病小鼠热刺激以及机械刺激疼痛的阈值,糖心安各剂量组降低糖尿病小鼠C纤维在强电压刺激下的高敏感性,糖心安0.15 g/kg、0.45 g/kg可改善Aδ纤维在强电刺激后易化程度。结论:糖心安改善糖尿病神经病变所产生的疼痛症状可能与其降低糖尿病小鼠C纤维在强电压刺激下的高敏感性,以及改善Aδ纤维在强电刺激后易化程度有关,但与血糖水平无明显关系,其详细作用机理还有待能进一步研究。
Objective: To evaluate the preventive and therapeutic effects of Tangxinan capsule on diabetic neuropathy. Methods: A single intraperitoneal injection of streptozotocin (150mg / kg) in diabetic mice was used to replicate the diabetic model. The diabetic mice were randomly divided into model group, carbamazepine 0.1 g / kg, g / kg, glycosaminoglycan 0.05 g / kg. Gavage given once a day for 14 days, the model group and normal control group were given equal volume of 0.5% sodium carboxymethyl cellulose, 1 hour after the last administration measured the heat pain threshold, tenderness threshold, blood glucose levels , Aδ fiber and C fiber sensitivity and other indicators. Results: The pain threshold and the threshold of mechanical stimulation in type Ⅰ diabetic mice decreased. Under CS condition (30V), C fibers were significantly reactive to electrical stimulation. Under the condition of post-CS (15V), Aδ fibers were strong After electro-stimulation, the degree of facilitation induced by low-level electrical stimulation decreased obviously; and the systolic and diastolic blood pressure of 0.15 g / kg and 0.45 g / kg significantly increased the threshold of thermal stimulation and mechanical stimulation in diabetic mice. High sensitivity of mouse C fiber under strong voltage stimulation, systolic blood pressure 0.15 g / kg and 0.45 g / kg can improve the facilitation of Aδ fiber after strong electrical stimulation. CONCLUSION: Symptoms and signs of systolic and diastolic dysfunction induced by diabetic neuropathy may be related to the high sensitivity of C-fiber in diabetic mice under high voltage stimulation and the facilitation of Aδ fiber after strong electrical stimulation. However, Obvious relationship, the detailed mechanism of action needs to be further studied.