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The Biological Seed Coating BIWANG 2 was prepared with PVA and SCMC as the binder，Na-Bentonite as the tackifier，potassium sorbate as antiseptic，1,2-Propylene glycol as antifreeze，they were mixed with Active Composition（Rs-5 and CL-8). The results showed that，after treatment with BIWANG 2，the germination rate of cotton was increased by 8.7％，and some Enzymatic activity，such as PAL，POD，PPO，SOD，and Photosnythesis pigment contents were improved. In addition，the results were also demonstrated that the ratios of K+/Na+，Ca2+/Na+，Mg2+/Na+ in cottons root they were 1.46 times，1.13 times and 1.76 times higher than that of CK,respectively. In cottons leaves，which were 3.26 times，2.99 times and 6.42 time shigher than that of CK. So，BIWANG 2 could reduce the adverse effects of salinity,promote cotton seedling growth，and improve the resistant of salt stressed cotton,which have a good applied merit.