【摘 要】
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Aim CD 133 is one of the most important cancer-initiating (stem) cell markers and was confirmed to be related to tumor self-renewal as well as metastatic potential.A novel method for monitoring cell p
【机 构】
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Department of Gastroenterology, Nanfang Hospital, Southern Medical University
【出 处】
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The 14th Congress of Gastroenterology China(第十四届全国消化系病学术会议)
论文部分内容阅读
Aim CD 133 is one of the most important cancer-initiating (stem) cell markers and was confirmed to be related to tumor self-renewal as well as metastatic potential.A novel method for monitoring cell proliferation both in vivo and intro with the membrane fluorescent dye DiO/DiD was used here to figure out the role of CD133 in resistance to chemotherapy of tumor cells.Methods We cultured DiO-stained HepG2 cancer cells for 10 days and isolated cells by flow cytometry based on expression levels of DiO/DiD.
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