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PURPOSE To evaluate the effect of pupil dilation on anterior chamber angle (ACA) measurements obtained by spectral-domain optical coherence tomography (SDOCT).METHODS A total of 40 eyes from 20 healthy, normal subjects underwent anterior segment OCT imaging using a Cirrus SD-OCT according to an IRB-approved protocol.For each eye, 5-line raster scans of the inferior angle were performed under 3 pupillary conditions: pupil constricted under standard room lighting, physiologic mydriasis in a darkened room, and after pharmacologic mydriasis).The inferior angle Schwalbes line-angle opening distance (SL-AOD) and the Schwalbes linetrabecular-iris space area (SL-TISA, measured 500 microns from Schwalbes line) were computed for each eye and pupillary condition.Measurements were performed by masked certified reading center graders using customized grading software.Differences in measurements between different pupillary conditions were compared.