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A phase detection technique for fluorescence-based optical chemical oxygen sensor is proposed.A phase of 0.01 degree can be distinguished to achieve 0.1% detected sensitivity of oxygen concentration.By simply subtracting the reference signal from the input signal, prior to lock-in amplifier ,the pure signal related to the phase difference can be extracted and amplify to increase the phase demodulation sensitivity.Experimental results validate the proposed technique, confirming it enormous possibilities in sensing application.