Calcineurin B subunit (CnB) exerts a synergistic effect with interferon-γ in vitro and in vivo by in

• 作 者: Zhenyi Su Lan Xu Wenlong Zhang Rui Yang Yanxia Yin Qun Wei
• 机 构: DepartmentofBiochemistryandMolecularBiology,BeijingNormalUniversity,GeneengineeringandBiotechnologyB
• 出 处: 第十一届全国酶学学术讨论会暨邹承鲁诞辰90周年纪念会
• 发表日期: 2013年5期
• 关键词: calcineurin B subunit IFN-γ synergy STAT1 integrin αM p38 MAP kinase

　　We have shown previously that the calcineurin B subunit (CnB) plays an important role in innate immunity, but little is known of the interactions between CnB and key cytokines.Here, we report that CnB and interferon-γ (IFN-γ) have a remarkable synergistic effect on typical IFN-γ response genes (e.g., CXCL9, CXCL10, iNOS, TRAIL) in both RAW264.7 macrophages and primary peritoneal macrophages.Further study showed that CnB strongly induced phosphorylation of STAT1 on Ser727 and had a weak effect on Tyr701, and the combination of CnB and IFN-γ caused more intense and rapid phosphorylation of STAT1, which may represent the fundamental basis of the synergy.