Identification of Animal Rabies in Inner Mongolia and Analysis of the Etiology Characteristics

• 作 者: YIN JingFeng TANG Qing WANG FengLong WANG JinLing DING YuLin TAO XiaoYan LI Hao SONG Miao GUO ZhenYang SHEN XinXin LIANG GuoDong
• 机 构: College of Veterinary Medicine,Inner Mongolia Agricultural University,Huhhot,010018,Inner Mongolia,C
• 出 处: 2013年中国畜牧兽医学会兽医病理学分会暨中国病理生理学会动物病理生理学专业委员会学术研讨会
• 发表日期: 2013年5期

　　Objective We performed pathological observation and etiological identification on specimens collected from cow, cattle and dog which were suspected of rabies in Inner Mongolia, in 2011, and analyzed their etiology and characteristics. Methods Pathological observation was conducted on the brain specimens of three infected animals with Hematoxylin-Eosin staining, followed by confirmation using immunofluorescence and nested RT-PCR methods. Finally, phylogenetic analysis was conducted using the virus N gene sequence amplified from three specimens. Results Eosinophilic and cytoplasmic inclusion bodies were seen in neuronai cells of the CNS; and rabies non-characteristic histopathological changes were also detected in the CNS. The three brain specimens were detected positive. N gene nucleotide sequence of these three isolates showed distinct sequence identity, therefore they fell into different groups in the phylogenetic analysis. N gene in the cow and dog had higher homology with that in Hebei isolate, but that in the beef cattle had higher homology with that in Mongolian lupine isolate and Russian red fox isolate. Conclusion Rabies were observed in the dairy cow, beef cattle and canine in the farm in Inner Mongolia, in 2011, which lead to a different etiology characteristics of the epidemic situation.