Dentification of differentially expressed genes in seeds of Brassica napus planted in Nanjing(altitu

• 作 者: Sanxiong Fu Cunkou Qi
• 机 构: Institute of Industrial Crops of Jiangsu Academy of Agricultural Sciences,Nanjing,210014,China
• 出 处: 中国作物学会油料作物专业委员会第七次会员代表大会暨学术年会
• 发表日期: 2013年12期
• 关键词: Brassica napus cDNA microarray Gene expression profiling Oil content Different a

　　Two Arabidopsis cDNA microarray were used to analyze the gene differential expression during seed development of a high oil Brassica napus, H105, planted in Nanjing (altitude : 8.9m) and Lhasa (altitude : 3658m), respectively.Oil contents of H105 were 46.04% ± 1.42% in Nanjing and 53.09% ± 1.35% in Lhasa.We isolated the seeds 30 days after flowering at periods preceding and including the major accumulation of storage oil.Transcript levels of 421 genes were altered twofold or more for H105 planted in Lhasa compared to that in Nanjing.In these 421 genes, 192 were up-regulated expression transcripts and 229 were down-regulated expression transcripts.These differentially expressed genes were involved in different processes and had different functions including metabolism, transporter, binding, transcription, structure, development, signal transduction, and other function-unknown etc.Some key genes, such as chlorophyll a-b binding protein, sucrose synthase, pyruvate kinase, 6-phosphogluconate dehydrogenase, ATP-citrate lyase, citrate synthase, isocitrate dehydrogenase, FAD6 and FAD7 etc relates to photosynthesis, sugar metabolism and fatty acid synthesis were identified differential expression.These results reveal the expressional disciplinarian of correlative genes and combine with results from others suggest that the improvement of photosynthesis and sugar metabolism perhaps can increase lipid biosynthesis.