弥猴结核IFN-γ体外释放检测法的建立与初步应用

来源 :第十四届中南地区实验动物科技交流会 | 被引量 : 0次 | 上传用户：winqstrong

【摘要】

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【作者】

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汪乾坤姜勇童胜涛郝丽影胡长敏陈颖钰李翔任习东陈焕春郭爱珍

【机构】

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华中农业大学农业微生物学国家重点实验室;华中农业大学动物医学院华中农业大学动物医学院

【出处】

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第十四届中南地区实验动物科技交流会

【发表日期】

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2014年3期

【关键词】

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猴结核猴IFN-γ 单克隆抗体 ELISA

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　　目的本研究旨在建立猴结核IFN-γ体外释放检测法,用于二猴结核病的特异性诊断.方法克隆表达了猕猴IFN-γ基因,利用纯化的重组IFN-γ免疫小鼠,获得12株高效价的单克隆抗体.用所获得的单克隆抗体及兔抗IFN-γ多克隆抗体建立了检测猴IFN-γ的夹心ELISA,检测灵敏度达到62.5 pg/mL.采集146份猕猴肝素钠抗凝全血,利用牛结核菌素(bPPD)和禽结核菌素(aPPD)体外刺激外周血淋巴细胞释放IFN-γ,用所建立的夹心ELISA及商品化试剂盒平行检测所有样本.结果该方法与皮内变态反应的符合率为84.93％,与进口试剂盒的符合率为93.84％.结论该方法具有良好的应用价值.

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弥猴结核IFN-γ体外释放检测法的建立与初步应用

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