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Objective To know the effect ofhepatectomy operation on circulating tum or cells of liver cancer patients during surgery.Methods Circulating blood was draw from 18 liver cancer patients before and after hepatectomy, 20 non-cancer patients during surgery and 20 healthy volunteers.Blood routine examination for leukocyte enumeration and kar yote counting by microscope were taken for every blood samples.Immunofluorescence and cell culture were used to identify the tumor cells and compare with the numbers and charac teristics of the circulating tumor cells in blood samples among all groups.Fluorescent anti bodies, AE1/AE3 and CD133 were used to mark tumor cells.Results The number of leu kocytes and karyotes in post-hepatectomy blood samples of liver cancer patients increased compared with pre-hepatectomy blood samples.The AE1/AE3 positive cells-samples were more than CD 133 positive cells-samples and double positive cells-samples(P<0.001).AE 1 /AE3 positive cells of healthy volunteers blood were more than post-hepatectomy blood of liver cancer patients(P<0.05).No significant differences in CD 133 positive cells and double positive cells were found among all groups(P > 0.05).There were no significant differences in clone formation rote after cell culture among all groups(P > 0.05).For 3 clone formed samples of liver cancer patients, there were no differences in tumor source, pathologic type and differentiation level.Conclusion The circulating blood of liver cancer patients, non-can cer patients and healthy people all exist circulating tumor cells.Hepatoma resection does not affect the number and characteristics of circulating tumor cells in liver cancer patients blood.