【摘 要】
：
Paclitaxel has been widely used in cancer therapy as a microtubule-targeted agent.Although, some imbalance of oxidation-antioxidation function has been observed in cancer cells treated with paclitaxel
【机 构】
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Department of Veterinary Surgery College of Veterinary Medicine Northeast Agricultural University Ch
【出 处】
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(BITs 3rd Annual World Cancer Congress-2010, Breast Cancer C
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Paclitaxel has been widely used in cancer therapy as a microtubule-targeted agent.Although, some imbalance of oxidation-antioxidation function has been observed in cancer cells treated with paclitaxel, the mechanisms and therapeutic implications remain unclear.In this article, the effect of paclitaxel was investigated on CHMm cells, a model of canine breast cancer cell line.CHMm cells were treated with paclitaxel in different concentrations and for different time.The cells viability, apoptotic morphology, reactive oxygen species (ROS), superoxide dismutase (SOD),catalase (CAT) and malondialdehyde (MDA) were detected at 24h, 48h and 72h after treatment.The cell viability was determined using the trypan blue exclusion assay.Apoptosis was analyzed using AO/EB staining.Apoptotic morphology was further observed with transmission electron microscope.The levels of ROS, SOD, CAT, and MDA were detected with kits.Together, the results suggested that the rates of apoptosis increased after paclitaxel treatment in a time and dose-dependent manner.This apoptosis displayed the typical apoptotic features.Contents of ROS and MDA were increased along with concomitant decrease in the activities of enzymic antioxidants (SOD, CAT), and levels were in a time and dose-dependent manner.In conclusion, the balance of oxidation-antioxidation function destroyed with decrease in the activities of enzymes antioxidants (SOD, CAT) induced by paclitaxel.The imbalance of oxidation-antioxidation function plays a major role in paclitaxel-induced reduction in the viability of CHMm cells.
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