【摘 要】
：
发育调节脑蛋白(developmentally regulation brain protein,Drebrin)是肌动蛋白丝(Actin filaments,F-actin)的主要调节蛋白,Dbn1是小鼠drebrin基因,目前只有Minghao Jin研究小组报道了Dbn1的基因组结构,染色体定位等,初步推测其功能是与细胞轴突生长和细胞迁移有关,但具体的调控机制还不清楚。神经干细胞(neura
【机 构】
：
组胚教研室,重庆400038 第三军医大学神经生物教研室
【出 处】
：
中国神经科学学会第四次会员代表大会暨第七届全国学术会议(The 7th Biennial Meeting and the
论文部分内容阅读
发育调节脑蛋白(developmentally regulation brain protein,Drebrin)是肌动蛋白丝(Actin filaments,F-actin)的主要调节蛋白,Dbn1是小鼠drebrin基因,目前只有Minghao Jin研究小组报道了Dbn1的基因组结构,染色体定位等,初步推测其功能是与细胞轴突生长和细胞迁移有关,但具体的调控机制还不清楚。神经干细胞(neural stem cells,NSCs)是主要来源于中枢神经系统,具有自我复制和分化为多种神经细胞的能力。目前在NSCs分化的细胞动力学以及分化和迁移前后神经元之间建立功能性信息连接、形成功能性神经网络机制等方面的研究进展颇微。本实验研究了Dbn1在NSCs分化过程中的表达模式和定位,在此基础上,进一步构建Dbn1真核表达载体和SiRNA载体转染NSCs,探讨Dbn1对NSCs体外分化的影响。结果与结论：1,Dbn1在小鼠神经干细胞非分化阶段的表达微弱,随着神经干细胞的分化Dbn1的表达逐步增强,其高峰在分化的中期(3 d左右),随着NSCs的分化成熟,Dbn1在细胞浆内的表达逐步集中于细胞突起,在分化后细胞的胞浆与突起上,Dbn1与actin表达有明显的共聚,提示Dbn1在NSCs分化过程中具体一定的作用,可能通过与actin结合调节NSCs分化以及突起的形成；2,过表达Dbn1对NSCs的分化状态以及突触蛋白1(synapsin1)的表达无明显影响,但上调突触后致密蛋白95(PSD-95)的表达；Dbn1 siRNA对NSCs分化状态以及synapsin1表达也无明显影响,但PSD-95表达明显下降。这些结果提示：Dbn1可能通过与actin结合影响NSCs的分化过程,通过调节PSD-95的表达水平,调节NSCs突起的形成。综上所述,Dbn1作为actin细胞骨架结合蛋白成员,参与调节NSCs分化过程中突起形成过程,并通过调节PSD-95的表达,可能对NSCs分化后突触的形成具有促进作用,但其具体机制还需进一步实验研究。
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