【摘 要】
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The use of allogenous or heterogeneous acellular tissue or organs for the repair of peripheral nerve injury has been frequently reported,but is limited by a series of problems,including limited source
【机 构】
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Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, Jiangsu Province, C
【出 处】
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第三届国际神经再生高峰论坛暨第五届脊髓损伤治疗与临床试验国际交流会(INRS2013 & 5th ISCITT)
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The use of allogenous or heterogeneous acellular tissue or organs for the repair of peripheral nerve injury has been frequently reported,but is limited by a series of problems,including limited sources,pathogen diffusion,and mechanical strength.This study aimed to develop a tissue-engineered nerve graft that not only preserves the superiority of acellular tissue,but also has overcomes the listed limitations.Schwann cells were cultured and adhered to the surface of a chitosan conduit/fibroin nerve scaffold,and vitamin C was used to promote Schwann cell secretion and deposition of extracellular matrix on the scaffold surface.Next,various cell constituents were removed by chemical methods,leaving only the extracellular matrix as a reconstructed Schwann cell extracellular matrix-modified tissue-engineered nerve graft.The reconstructed tissue-engineered nerves were used to bridge 10 mm rat sciatic nerve defects.Allogenous acellular sciatic nerve was used as the positive control,and non-modified nerve scaffolds were used as the negative control.A large amount of extracellular matrix was deposited on the surface of Schwann cell extracellular matrix-modified tissue-engineered nerve grafts,and immunofluorescence staining confirmed the presence of fibronectin and laminin.Schwann cell extracellular matrix-modified tissue-engineered nerve grafts,acellular allogenous sciatic nerves,and chitosan conduit and fibroin scaffolds were used to bridge 10 mm adult rat sciatic nerve defects.At postoperative 12 weeks,the compound muscle action potential,wet weight ratio of target muscles,myelin sheath thickness,neuromuscular junction,and sciatic nerve index were evaluated.The results showed that Schwann cell extracellular matrix modified tissue-engineered nerve grafts better repair peripheral nerve injury.Taken together,these data suggest that tissue-engineered nerve grafts constructed from a chitosan conduit/fibroin scaffold modified by a layer of Schwann cell-secreted bioactive extracellular matrix are more beneficial for nerve injury repair than standard methods,providing a novel approach for peripheral nerve regeneration.
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