【摘 要】
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The epidemiological studies of peripheral nerve injuries (PNIs) have shown that the incidence of PNIs may vary according to periods of peace or wartime.Although peripheral nerves have an intrinsic abi
【机 构】
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Department of Bums, Second Affiliated Hospital of Kunming Medical University, Bum Institute of Yunna
【出 处】
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第三届国际神经再生高峰论坛暨第五届脊髓损伤治疗与临床试验国际交流会(INRS2013 & 5th ISCITT)
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The epidemiological studies of peripheral nerve injuries (PNIs) have shown that the incidence of PNIs may vary according to periods of peace or wartime.Although peripheral nerves have an intrinsic ability to regenerate following injury,PNIs in humans often result in incomplete functional recovery.Therefore,it is necessary to develop novel therapeutic strategies.The aim of this study was to demonstrate the ability and determine the effect of delivering the LacZ gene as a reporter to dorsal root ganglion by inoculating mouse sciatic nerve with a tetracycline-regulated,replication-defective herpes simplex virus type I (HSV-1) vector (QR9TO-LacZ).Female CD1 mice,8-10 weeks old,were randomly divided into a doxycycline diet group (QR9-Dox) and a control group.Mice were fed a doxycycline (6 g/kg) or standard diet for 7 days prior to inoculation.On the day of inoculation,mice were weighed and anesthetized with 50 mg/kg pentobarbital.Sciatic nerves were injected bilaterally with 5 μL (1 106 PFU) of QR9TO-LacZ vector (QR9-Dox group) or 5 uL of diluent (control group).After inoculation,mice were fed ad libitum either a standard or a doxycycline-containing diet.The sciatic nerves,dorsal root ganglions,and lumbar spinal cord (L3 6) were harvested at 3 days post-inoculation to detect the activity of p-galactosidase with X-gal staining.In the control group,all observed nervous tissues were negative for p-galactosidase activity.In contrast,the QR9-Dox group showed positive staining in both sciatic nerves and dorsal root ganglions (83.3%,P< 0.05),as well as in lumbar spinal cord (33.3%,P > 0.05).This study demonstrates that the LacZ gene,as a reporter,can be delivered to dorsal root ganglions through inoculation of the sciatic nerve with a QR9TO-LacZ viral vector,and that the gene expression can be effectively controlled with tetracycline.
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