【摘 要】
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The male reproductive toxicity of endosulfan has been proved.Nevertheless, the underlying molecular mechanisms of the apoptosis caused by endosulfan in spermatogenic cells remain poorly understood.In
【机 构】
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Department of Toxicology and Hygienic Chemistry, School of Public Health, Capital Medical University
【出 处】
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第六届中国毒理学会免疫毒理专业委员会换届及学术会议
论文部分内容阅读
The male reproductive toxicity of endosulfan has been proved.Nevertheless, the underlying molecular mechanisms of the apoptosis caused by endosulfan in spermatogenic cells remain poorly understood.In order to investigate the reproductive toxicity mechanism caused by endosulfan, There were four gourps which had 8 wistar male rats randomly, and the rats in different groups received different dose endosulfan respectively for a period of 21 days.GC-1 spermatogenic cells line were divided into four groups, and exposed to different dose endosulfan for 24 hours.The research showed that endosulfan decreased the cell viability, damaged cell membrane and induced the apoptosis in spermatogenic cell;Endosulfan obviouly activated the protein expression of PKC-δ, p53, p21cip1, p27kip1,Fas, FasL, Caspase-8, Caspase-3, and inhibited the expression of E2F-1;endosulfan also induced oxidative stress and DNA damage in spermatogenic cells.The research suggested that endosulfan could lead to E2F-1-induced apoptosis of spermatogenic cells by activating the negative regulation factors of cell cycle, and endosulfan might cause the apoptosis by death receptor pathway caused oxidative stress.
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